Shiraia bambusicola, isolation and analysis of genetic diversity of fungi

The outer (Shiraia bambusicola p. Henn.) Is a rare type of Parasitic fungi in our country, have great medicinal value, with the outer bamboo red with photosensitive activity in Streptozotocin (bamboo bamboo Red Red armillarisin a and strain b) discovery of application research on shiraia bambusicola henn and more attention. In order to understand the outer geographic genetic differentiation among populations using ISSR molecular marker for Jiangsu, Anhui and Zhejiang 3 8 populations in the province a total of 107 outer samples for genetic diversity analysis. This study probes Mg2+, dNTPs and Taq DNA polymerase, primers and template DNA, 5 factors that influence on the expansion of ISSR-PCR, based on single-factor test and orthogonal design method